Allspin

AllspinTM total DNA/RNA purification kit provides a convenient method for the isolation of total DNA and total RNA simultaneously from a single sample of tissue or cultured cells. DNA and RNA are purified separately from a same sample by individual but successive procedure using column B and column W respectively. Whole procedure can be performed in just 30 minutes and the length of obtained DNA is up to 50 kb (average is 30 kb) and that of RNA is longer than 200 nucleotides.

Features and Benefits

? Glassfiber membrane technology
? Sample size : Up to 20 mg tissue or up to 1 x 10⁷ cultured cells
? Typical yield of RNA : Up to 20 ? / 1 x 10⁶ cultured cells Up to 60 ? / 10 mg liver tissue
? Typical yield of DNA : Up to 10 ? / 1 x 10⁶ cultured cells Up to 25 ? / 10 mg liver tissue
? High purity
? Preparation time : ~ 30 minutes
? Stable and consistent yield
? No phenol / chloroform extraction
? No ethanol precipitation
? Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning, real-time PCR, RNase protection assays and other analytical procedures