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The ExgeneTM Viral DNA/RNA kit provides fast and easy methods for the purification of total nucleic acids from viral samples such as cell-free fluid, cell-cultrue supernatant, plasma, serum, swab, urine, and virus-infected samples. Purified nucleic acids can be used directly for PCR, qPCR, RT-PCR, or any downstream application without further manipulation.
ExgeneTM Viral DNA/RNA kit utilizes the advanced silica-binding technology to purify total nucleic acids sufficiently pure for many applications. Viral samples are lysed in optimized buffer containing detergent and lytic enzyme. Under optimized binding condition, nucleic acids in the lysate bind to silica membrane and impurities pass through membrane into a collection tube. The membranes are washed with a series of alcohol-containing buffer to remove any traces of proteins, cellular debris and salts. Finally pure nucleic acids are released into a clean collection tube with deionized water or low ionic strength buffer. The eluate should be treated with care because nucleic acids are very sensitive to contaminants, such as nucleases, often found on general labware and dust. To ensure nucleic acids stability, it is recommended to store at 4°C for immediate analysis or to freeze at -70°C for long-term storage.
Features and Benefits
– Spin column format
– Stable and consistent yield
– Preparation time : ~ 20 minutes
– No phenol / chloroform extraction
– No ethanol precipitation
– Micro column & carrier RNA enhance the performance of viral
– Various viral samples : cell-free fluid, cell-culture supernatant,
plasma, serum, swab, urine and virus-infected samples
– Ready for use in PCR, RT-PCR, real-time PCR and other analytical