Ribospin

RibospinTM provides a convenient method for isolation of total RNA from cell and tissue samples. RibospinTM procedures employed the glassfiber membrane technology for the fastest and the most convenient of high purity RNA isolation, instead of conventional alcohol precipitation or phenol / chloroform extraction. Whole procedure takes only 15 minutes and the eluates are suitable for RT-PCR or any downstream application without further manipulation.

Features and Benefits

? Glassfiber membrane technology
? Sample size : Up to 25 mg tissue or up to 5 x 106 cultured cells
? Typical yield : Up to 20 ? / 1 x 106 cultured cells
Up to 60 ? / 10 mg liver tissue
? High purity : A260/A230 > 2.0, A260/A280 > 1.8
? Preparation time : ~ 15 minutes
? Stable and consistent yield
? No phenol / chloroform extraction
? No ethanol precipitation
? Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning,
real-time PCR, RNase protection assays and other analytical procedures